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unmethylated gpppg  (New England Biolabs)


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    Structured Review

    New England Biolabs unmethylated gpppg
    Unmethylated Gpppg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unmethylated+gpppg/G(5)ppp(5)G+RNA+Cap+Struc+Analog/pm42103131-88-26-28
    Average 95 stars, based on 189 article reviews
    unmethylated gpppg - by Bioz Stars, 2026-11
    95/100 stars

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    Concentration Assay:

    Article Title: Translational hub ribosomal protein S5 promotes glioblastoma progression by affecting translation patterns.
    Article Snippet: In vitro transcription, capping, and polyadenylation of the monocistronic and bicistronic reporter RNAs were performed using the HiScribe T7 ARCA mRNA Kit (NEB, Catalog # E2060S) following the manufacturer’s instructions, with 1 μg of linearized DNA template per 20 μL reaction. .. To generate mRNAs with a nonfunctional cap, transcription was carried out using RiboMAXTM Large Scale RNA Production Systems (Promega, Catalog # P1280) in the presence of unmethylated GpppG (NEB, Catalog # S1407S) at a final concentration of 8 mM, followed by the same polyadenylation step. .. To generate digoxigenin (DIG)-labeled RNA probes, the pGEM-3zf vector was linearized with EcoRI, followed by in vitro transcription using SP6 RNA polymerase with the DIG Northern Starter Kit (Roche, Catalog #12039672910).



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    95
    New England Biolabs unmethylated gpppg
    Unmethylated Gpppg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unmethylated+gpppg/G(5)ppp(5)G+RNA+Cap+Struc+Analog/pm42103131-88-26-28
    Average 95 stars, based on 1 article reviews
    unmethylated gpppg - by Bioz Stars, 2026-11
    95/100 stars
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